In a dideoxy DNA sequencing experiment, four separate reactions a... | Study Prep in Pearson
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In a dideoxy DNA sequencing experiment, four separate reactions a... | Study Prep in Pearson
In a dideoxy DNA sequencing experiment, four separate reactions are carried out to provide the replicated material for DNA sequencing gels. Reaction products are usually run in gel lanes labeled A, T, C, and G.Identify the nucleotides used in the dideoxy DNA sequencing reaction that produces molecules for the A lane of the sequencing gel.
Jul 23, 2022
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SOLVED: Dideoxy DNA sequencing is based on chain termination with dideoxynucleotides. Why are normal deoxynucleotides also included in the reaction?to provide a 2'-OH (hydroxyl) required for incorporation of the dideoxynucleotidesto allow production of range of synthesis products of different lengthsto act as primer for chain termination by the dideoxynucleotidesto provide a template for DNA polymerase
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